heatmap graph graphpad prism version 10.1.2 for windows  (GraphPad Software Inc)

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Chronic unpredicted mild stress effects in female and male ALS-predisposed mice. A-B. Rotarod and weight results in female h SOD1 G93A are showed at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per group, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Rotarod of female h SOD1 G93A STRESSED at end point vs. female h SOD1 G93A STRESSED at starting point ** p < 0.01; weight of female h SOD1 G93A CTRL at end point vs. female h SOD1 G93A CTRL at starting point * p < 0.05. C-D. Rotarod and weight results in male h SOD1 G93A are described at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per groups, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Weight of male h SOD1 G93A CTRL at end point vs. male h SOD1 G93A CTRL at starting point ** p < 0.01. E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups). Results are showed as red-to-green scale, using data of three mice per groups and indicating 1 (black) as baseline reference point of female or male h SOD1 G93A CTRL (not shown). Two-way ANOVA followed by Sidák’s multiple comparison test was used as statistical analysis and the significantly results were listed in Table .

Article Snippet: Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions.

Techniques: Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Gene expression and relative PPI in CTRL and STRESSED cells. (A) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows relative gene expression of NSC-34 h SOD1 WT STRESSED, NSC-34 h SOD1 G93A CTRL and STRESSED, normalized to NSC-34 h SOD1 WT CTRL. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT CTRL (not shown). Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT (not shown). Unpaired t-test statistical analysis for each gene was used: NSC-34 h SOD1 G93A CTRL vs. NSC-34 h SOD1 WT CTRL, NSC-34 h SOD1 G93A STRESSED vs. NSC-34 h SOD1 WT STRESSED. C-D. Bar plot graphs show the genes significantly up-regulated and/or down-regulated, based on the unpaired t-test statistical analysis previously described in CTRL and STRESSED conditions. E-F. PPI, performed by STRING analysis, shows interactions among predicted proteins in CTRL and STRESSED conditions.

Article Snippet: Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions.

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Chronic unpredicted mild stress effects in female and male ALS-predisposed mice. A-B. Rotarod and weight results in female h SOD1 G93A are showed at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per group, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Rotarod of female h SOD1 G93A STRESSED at end point vs. female h SOD1 G93A STRESSED at starting point ** p < 0.01; weight of female h SOD1 G93A CTRL at end point vs. female h SOD1 G93A CTRL at starting point * p < 0.05. C-D. Rotarod and weight results in male h SOD1 G93A are described at starting and end point in STRESSED and CTRL (not stressed) groups. Results are represented as median and quartile range of three mice per groups, considering the average of three trials. Mixed-effects analysis followed by Uncorrected Fisher’s LDS analysis was used for statistical results. Weight of male h SOD1 G93A CTRL at end point vs. male h SOD1 G93A CTRL at starting point ** p < 0.01. E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups). Results are showed as red-to-green scale, using data of three mice per groups and indicating 1 (black) as baseline reference point of female or male h SOD1 G93A CTRL (not shown). Two-way ANOVA followed by Sidák’s multiple comparison test was used as statistical analysis and the significantly results were listed in Table .

Article Snippet: E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups).

Techniques: Gene Expression, Comparison

Journal: Scientific Reports

Article Title: Stress exposure affects amyotrophic lateral sclerosis pathogenesis via PI3K/Akt and focal adhesion pathways: evidence from three experimental models

doi: 10.1038/s41598-025-02167-9

Figure Lengend Snippet: Gene expression and relative PPI in CTRL and STRESSED cells. (A) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows relative gene expression of NSC-34 h SOD1 WT STRESSED, NSC-34 h SOD1 G93A CTRL and STRESSED, normalized to NSC-34 h SOD1 WT CTRL. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT CTRL (not shown). Two-way ANOVA followed by Tukey’s multiple comparisons test was used as statistical analysis and the details were listed in Table . (B) Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative expression of genes of interest, in both CTRL and STRESSED experimental conditions. Results are showed as red-to-green scale of nine independent experiments, indicating 1 (black) as baseline of NSC-34 h SOD1 WT (not shown). Unpaired t-test statistical analysis for each gene was used: NSC-34 h SOD1 G93A CTRL vs. NSC-34 h SOD1 WT CTRL, NSC-34 h SOD1 G93A STRESSED vs. NSC-34 h SOD1 WT STRESSED. C-D. Bar plot graphs show the genes significantly up-regulated and/or down-regulated, based on the unpaired t-test statistical analysis previously described in CTRL and STRESSED conditions. E-F. PPI, performed by STRING analysis, shows interactions among predicted proteins in CTRL and STRESSED conditions.

Article Snippet: E. Heatmap graph (created using GraphPad Prism version 10.1.2 for Windows) shows the relative gene expression in motor cortex and lumbar spinal cord samples of STRESSED groups normalized to CTRL (distinguishing female and male groups).

Techniques: Gene Expression, Expressing

Journal: bioRxiv

Article Title: The mitochondrial NAD transporter SLC25A51 is a modulator of beta cell senescence and type 2 diabetes

doi: 10.1101/2025.04.09.647991

Figure Lengend Snippet: (A) ( A ) A diagram depicting the generation of SLC25A51-floxed C57BL/6N mice using a CRISPR-Cas9-based approach by Biocytogen Pharmaceuticals. ( B, C ) SLC25A51 expression per cell was compared between T2D patients and healthy controls using two different human beta-cell scRNA-seq datasets (E-MTAB-5061, GSE81608) ( Table S3 ). Heatmaps of Cdkn1a (p21) and Cdkn2a (p16) expression were also analyzed in both datasets. ( D ) 30-week-old SLC25A51-floxed C57BL/6N male mice were infused with either AAV6-INS1-Empty or AAV6-INS1-CRE viruses via pancreatic ductal infusion. Mice were sacrificed 15 weeks post-surgery. SLC25A51, p16 INK4A , MTCO2, and beta-actin protein expression levels were analyzed in pancreatic beta cells isolated from 45-week-old INS1-EMPTY (n=2) and INS1-CRE (n=4) groups. ( E-K ) Pancreatic ductal infusion of either PBS, AAV6-INS1-Empty, or AAV6-INS1-CRE was performed in each group of female C57BL/6N mice (n=5–6 per group). ( F ) Random blood glucose levels and ( G ) body weight were measured twice a week, and ( H ) beta-hydroxybutyrate levels were measured once a week for each mouse. ( I-K ) Six or eight weeks post-surgery, female mice underwent an IPGTT and ITT. Graphs show blood glucose levels at ( I, J ) each time point and area under the curve. ( I ) During the IPGTT, serum was collected from each group at 0 and 15 min to measure ( K ) serum insulin levels. Two weeks after the IPGTT, ( J ) an ITT was performed to test insulin sensitivity. Two-way ANOVA; error bars represent SEM; **p < 0.01.

Article Snippet: The data were visualized using heatmaps or dot-plot graphs in GraphPad Prism.

Techniques: CRISPR, Expressing, Isolation

Journal: Pharmaceuticals

Article Title: Computational Investigation of Montelukast and Its Structural Derivatives for Binding Affinity to Dopaminergic and Serotonergic Receptors: Insights from a Comprehensive Molecular Simulation

doi: 10.3390/ph18040559

Figure Lengend Snippet: Structural alignment and similarity analysis of Montelukast and its modifications. ( A ) Superimposition of Montelukast modifications with the native structure (backbone representation). ( B ) A 3D superimposition of Montelukast modifications with the native structure. Different colors represent different Montelukast modifications, allowing visual comparison of structural variations. ( C ) Pairwise similarity correlation heatmap, depicting the structural similarity scores between Montelukast and its modified derivatives. Higher similarity scores indicate minimal structural deviation, while lower scores suggest significant modifications. ( D ) Similarity score distribution plot, providing a comparative analysis of the degree of structural resemblance between each modified derivative and the native Montelukast. Purple colors indicate the similarity of each modification compared to the native structure of Montelukast.

Article Snippet: The similarity data were processed and visualized as a heatmap graph using OriginLab Pro version 2024 (OriginLab Corporation, Northampton, MA, USA) [ ].

Techniques: Comparison, Modification